Robin Green

Ph.D. Candidate
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Present title and affiliations:
Ph.D. Candidate
Division of Basic Sciences
Fred Hutchinson Cancer Research Center
Molecular and Cellular Biology Program
University of Washington

Advisor: Wenying Shou

Address:
Fred Hutchinson Cancer Research Center
1100 Fairview Ave N.
A2-168
Seattle,WA 98109



Education
University of Washington
Ph.D. in Molecular and Cellular Biology
Expected Graduation: December 2017
Michigan State University
BS with Honor in Biochemistry and Molecular Biology
Minor in Computer Science
Honors College Member 2008-2012



Research Experience
Fred Hutchinson Cancer Research Center: Graduate Research Assistant, Laboratory of Wenying Shou, May 2013-Present
Michigan State University: Undergraduate Research Assistant,Laboratory of Eric Hegg , August 2008-August 2012
Merck Sharpe & Dohme: Molecular Biology Intern Basic Vaccines Research Division, May 2011-August 2011



Publications (Peer Reviewed)

Cornish AJ , Green R , Gärtner K, Mason S, Hegg EL Characterization of Hydrogen Metabolism in the Multicellular Green Alga Volvox carteri ". PLoS One. 2015 Apr 30;10(4):e0125324. doi: 10.1371/journal.pone.0125324. eCollection 2015 PMID: 25927230



Publications (Book Chapters)

Green R , Shou W "Modeling community population dynamics with the open-source language R" . Methods Mol Biol. 2014;1151:209-31. doi: 10.1007/978-1-4939-0554-6_15. PMID: 24838889 [ PDF]



Research Funding and Fellowships
National Science Foundation (NSF) Graduate Research Fellowship, 2013-Present


Selected Professional Presentations

2016: Starvation-induced Niche Construction Facilitates the Evolution of Metabolic Dependence, 2016 BEACON Congress, East Lansing, MI Selected Talk

2016: Starvation-induced Niche Construction Facilitates the Evolution of Metabolic Dependence, 2nd ASM Conference on Experimental Microbial Evolution, Washington DC Poster

2016: In stressful times, make new friends: niche construction facilitates the evolution of metabolic dependence, 2016 Evolution in Oregon, Washington, Idaho, and British Columbia (EVO-WIBO), Port Townsend, WA Selected Talk

2015: In stressful times, make new friends: niche construction facilitates the evolution of metabolic dependence, Fred Hutchinson Cancer Research Center Basic Sciences Mini-Symposium, Seattle, WA Selected Talk


Additional Selected Honors

2016: NSF BEACON Travel Award ($500)

2015: NSF BEACON Travel Award ($500)

2015: Society for the Study of Evolution Travel Award ($500)

2014: NSF BEACON Travel Award ($500)

2014: ASM Graduate Student Travel Award (to attend 1st ASM Conference on Experimental Microbial Evolution) ($500)



Academic Service Positions

2016: Evolution in Oregon, Washington, Idaho, and British Columbia (EVO-WIBO)
Organizing Committee Member

Additional Outreach

2016-Present: American Civil Liberties Union of Washington State, Seattle, WA
Data Analyst

Wetlab Skill Sets

Molecular Biology - DNA/RNA isolation, Gel Electrophoresis, Plasmid Construction, Gibson Assembly, PCR, Standard Molecular Cloning and Subcloning, Site-Directed Mutagenesis

Quantitative Molecular Biology - qPCR and qRT-PCR (Taqman probe based)

Next Generation Sequencing - library preparation by Nextera protocols, RADSeq

Flow Cytometry (strong proficiency) - FlowJo Analysis, Cell Population Quantitation, Differential Staining

HPLC (strong proficiency) - Quantitative Metabolite Profiling by UV-Vis and Fluorescence, Sample Derivatization and Stabilization

Cellular Metabolite Profiling - Metabolism Quenching and Metabolite Extraction

Continuous Culturing (strong proficiency) - Long-term Chemostat and Turbidostat Culturing

Quantitative Microscopy (strong proficiency) - Developed automated microscopy assays for assessing cell growth and viability

Quantitative Plate Model Bioassay Development (strong proficiency) - Developed yeast based biosensors for the quantitation of specific metabolites with high reproducibility

Computational Skill Sets

Next Generation Sequencing Analysis - SNP and indel detection, ploidy analysis, and copy-number variation detection via custom written sequence analysis pipeline

R (strong proficiency) - Scripting, statistical analysis, figure generation with ggplot2, RMarkdown, Bioconductor

Python (strong proficiency) - Scripting, data analysis, scipy/numpy, pandas, Biopython

Unix - Scripting and standard operations

Software Version Control - Github

Additional Data Analysis and Visualization - SQL, Tableau, Excel